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Anti-Influenza A H1N3 Hemagglutinin/HA Antibody (5F639) 是一种 Mouse 抗体,靶向 Influenza A H1N3 Hemagglutinin/HA。Anti-Influenza A H1N3 Hemagglutinin/HA Antibody (5F639) 可用于 WB,ELISA。
Anti-Influenza A H1N3 Hemagglutinin/HA Antibody (5F639) 是一种 Mouse 抗体,靶向 Influenza A H1N3 Hemagglutinin/HA。Anti-Influenza A H1N3 Hemagglutinin/HA Antibody (5F639) 可用于 WB,ELISA。
产品描述 | Anti-Influenza A H1N3 Hemagglutinin/HA Antibody (5F639) is a Mouse antibody targeting Influenza A H1N3 Hemagglutinin/HA. Anti-Influenza A H1N3 Hemagglutinin/HA Antibody (5F639) can be used in WB,ELISA. |
Ig Type | Monoclonal Mouse IgG1 |
克隆号 | 5F639 |
应用 | WB,ELISA |
推荐剂量 | WB: his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect H1N3-HA in WB. Using a DAB detection system, the detection limit for H1N3-HA is approximately 20 ng/lane under non-reducing conditions and reducing conditions.; ELISA: 1:1000-1:2000 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H1N3 Hemagglutinin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
偶联 | Unconjugated |
免疫原 | Recombinant H1N3 HA protein |
抗原种属 | H1N3 |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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