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Anti-IL-32 alpha/IL32A Antibody (2S286) 是一种 Mouse 抗体,靶向 IL-32 alpha/IL32A。Anti-IL-32 alpha/IL32A Antibody (2S286) 可用于 WB。
Anti-IL-32 alpha/IL32A Antibody (2S286) 是一种 Mouse 抗体,靶向 IL-32 alpha/IL32A。Anti-IL-32 alpha/IL32A Antibody (2S286) 可用于 WB。
产品描述 | Anti-IL-32 alpha/IL32A Antibody (2S286) is a Mouse antibody targeting IL-32 alpha/IL32A. Anti-IL-32 alpha/IL32A Antibody (2S286) can be used in WB. |
Ig Type | Monoclonal Mouse IgG2a |
克隆号 | 2S286 |
交叉反应 | Human |
验证活性 | Anti-IL32A mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:30 kDa(We are unsure as to the identity of these extra bands.) |
应用 | WB |
推荐剂量 | WB: 1:500-1:1000 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-32 alpha / IL32A (rh IL-32 alpha / IL32A; TMPY-01033; NP_001012651.1; Met1-Lys131). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer. |
偶联 | Unconjugated |
免疫原 | Recombinant Human IL-32 alpha / IL32A Protein (TMPY-01033) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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