Anti-IkB alpha/NFKBIA Antibody (5M346) is a Mouse antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Antibody (5M346) can be used in WB,IHC-P,IHC-Fr,IF,ICC/IF.

IgG1

5M346

Human,Rat

1. Sample:
Jurkat (Human) Cell Lysate at 30 μg
Primary: Anti-IKB alpha (TMAB-00927) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
2. Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 μg
Lane 2: Hela (Human) Cell Lysate at 30 μg
Lane 3: Siha (Human) Cell Lysate at 30 μg
Primary: Anti-IKB alpha (TMAB-00927) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
3. Sample:
293t (Human) Cell Lysate at 30 μg
Primary: Anti-IKB alpha (TMAB-00927) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
4. Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IKB alpha) Monoclonal Antibody, Unconjugated (TMAB-00927) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
5. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (IKB alpha) monoclonal Antibody, unconjugated (TMAB-00927) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ICC/IF: 1:50-1:200

Monoclonal

Recombinant Protein: human IKB alpha Protein

Human

NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway