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Anti-IGF2/IGF-II Antibody (5T527) 是一种 Rabbit 抗体,靶向 IGF2/IGF-II。Anti-IGF2/IGF-II Antibody (5T527) 可用于 WB,ICC/IF,IHC。
Anti-IGF2/IGF-II Antibody (5T527) 是一种 Rabbit 抗体,靶向 IGF2/IGF-II。Anti-IGF2/IGF-II Antibody (5T527) 可用于 WB,ICC/IF,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-IGF2/IGF-II Antibody (5T527) is a Rabbit antibody targeting IGF2/IGF-II. Anti-IGF2/IGF-II Antibody (5T527) can be used in WB,ICC/IF,IHC. |
别名 | 胰岛素样生长因子 |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of IGF2 on human placenta lysates using anti-IGF2 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-IGF2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IGF2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IGF2 antibody. Counter stained with hematoxylin. 5. ICC staining IGF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining IGF2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining IGF2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining IGF2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:100-500 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | IGF-II (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) is a secreted 8 kDa polypeptide that belongs to the insulin family of peptide growth factors. It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation. In human, IGF-II is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF-II levels drop after the perinatal period, an effect attributed to the lack of a key gene promoter. This may indicate that postnatally, IGF-II has either a limited, or local effect only in rodent. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 11 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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