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Anti-HGPRT Antibody (2V553)
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产品编号 TMAC-02013
别名 Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1, HPRT, HGPRTase, HGPRT
Anti-HGPRT Antibody (2V553) 是一种 Rabbit 抗体，靶向 HGPRT。Anti-HGPRT Antibody (2V553) 可用于 WB,IHC,IP。
Anti-HGPRT Antibody (2V553)
Anti-HGPRT Antibody (2V553)
还可以产品编号 TMAC-02013 别名 Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1, HPRT, HGPRTase, HGPRT
Anti-HGPRT Antibody (2V553) 是一种 Rabbit 抗体，靶向 HGPRT。Anti-HGPRT Antibody (2V553) 可用于 WB,IHC,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-HGPRT Antibody (2V553) is a Rabbit antibody targeting HGPRT. Anti-HGPRT Antibody (2V553) can be used in WB,IHC,IP. |
| 别名 | Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1, HPRT, HGPRTase, HGPRT |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat,zebrafish |
| 验证活性 | 1. Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution. Positive control:Lane 1: Hela, Lane 2: A431, Lane 3: MCF-7, Lane 4: 293, Lane 5: Rat kidney, Lane 6: Rat brain, Lane 7: Mouse testis, Lane 8: Mouse colon. 2. Western blot analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution. 3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; IP: 1:10-50 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism, HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive self-mutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 25 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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