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Anti-HDAC2 Antibody (4L672)
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产品编号 TMAH-00534
别名 Histone deacetylase 2, HDAC 2, HD2, EC 3.5.1.98
Anti-HDAC2 Antibody (4L672) 是一种抗体，靶向 HDAC2。Anti-HDAC2 Antibody (4L672) 可用于 ELISA, WB, IHC。
Anti-HDAC2 Antibody (4L672)
Anti-HDAC2 Antibody (4L672)
还可以产品编号 TMAH-00534 别名 Histone deacetylase 2, HDAC 2, HD2, EC 3.5.1.98
Anti-HDAC2 Antibody (4L672) 是一种抗体，靶向 HDAC2。Anti-HDAC2 Antibody (4L672) 可用于 ELISA, WB, IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,310 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-HDAC2 Antibody (4L672) is an antibody targeting HDAC2. Anti-HDAC2 Antibody (4L672) can be used in ELISA, WB, IHC. |
| 别名 | Histone deacetylase 2, HDAC 2, HD2, EC 3.5.1.98 |
| Ig Type | Rabbit IgG |
| 克隆号 | 4L672 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate -All lanes: HDAC2 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 56, 52 kDa -Observed band size: 60 kDa 2. IHC image of TMAH-00534 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| 应用 | |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. Cytoplasm. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human HDAC2 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Cardiovascular, Stem cells |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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