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Anti-GPC3 Antibody (4L576) 是一种抗体,靶向 GPC3。Anti-GPC3 Antibody (4L576) 可用于 ELISA, WB, IF。
Anti-GPC3 Antibody (4L576) 是一种抗体,靶向 GPC3。Anti-GPC3 Antibody (4L576) 可用于 ELISA, WB, IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,305 | 5日内发货 | |
100 μL | ¥ 2,190 | 5日内发货 |
产品描述 | Anti-GPC3 Antibody (4L576) is an antibody targeting GPC3. Anti-GPC3 Antibody (4L576) can be used in ELISA, WB, IF. |
别名 | SGBS1, SGBS, SGB, SDYS, OCI-5, MXR7, GTR2-2, Glypican 3, DGSX |
Ig Type | Rabbit IgG |
克隆号 | 4L576 |
交叉反应 | Human |
验证活性 | 1. Western Blot -Positive WB detected in: 293 whole cell lysate, K562 whole cell lysate -All lanes: GPC3 antibody at 0.9μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 66, 60, 69 KDa -Observed band size: 66 KDa 2. Immunofluorescence staining of HepG2 cells with TMAH-00502 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
应用 | |
推荐剂量 | WB:1:500-1:5000; IF:1:20-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Cell surface proteoglycan that bears heparan sulfate. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human GPC3 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Stem Cells |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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