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Anti-Glutaminase Antibody (8G424)

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货号 TMAC-01686

别名 L-glutamine amidohydrolase, KIAA0838, K-glutaminase, KGA, Glutaminase kidney isoform, mitochondrial, GLSK, GLS, GDPAG, GAM, GAC, EIEE71, EC:3.5.1.2, DEE71, CASGID, AAD20

Anti-Glutaminase Antibody (8G424) 是一种 Rabbit 抗体,靶向 Glutaminase。Anti-Glutaminase Antibody (8G424) 可用于 ICC/IF,IHC,WB。

Anti-Glutaminase Antibody (8G424)

Anti-Glutaminase Antibody (8G424)

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Rating icon 还可以
货号 TMAC-01686 别名 L-glutamine amidohydrolase, KIAA0838, K-glutaminase, KGA, Glutaminase kidney isoform, mitochondrial, GLSK, GLS, GDPAG, GAM, GAC, EIEE71, EC:3.5.1.2, DEE71, CASGID, AAD20

Anti-Glutaminase Antibody (8G424) 是一种 Rabbit 抗体,靶向 Glutaminase。Anti-Glutaminase Antibody (8G424) 可用于 ICC/IF,IHC,WB。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍


生物活性
产品描述
Anti-Glutaminase Antibody (8G424) is a Rabbit antibody targeting Glutaminase. Anti-Glutaminase Antibody (8G424) can be used in ICC/IF,IHC,WB.
别名L-glutamine amidohydrolase, KIAA0838, K-glutaminase, KGA, Glutaminase kidney isoform, mitochondrial, GLSK, GLS, GDPAG, GAM, GAC, EIEE71, EC:3.5.1.2, DEE71, CASGID, AAD20
Ig Type
IgG
克隆号
8G424
反应种属
Human,Mouse,Rat
验证活性
1. Western blot analysis of Glutaminase on different lysates using anti-Glutaminase antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutaminase antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutaminase antibody. Counter stained with hematoxylin.
4. ICC staining Glutaminase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Glutaminase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用ICC/IFIHCWB
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Glutamine is an important molecule involved in several cellular functions, including nitrogen and carbon transport, hepatic urea synthesis, renal ammoniagenesis, and gluconeogenesis. Glutamine is catabolized by either the liver-type (LGA) or kidney-type (KGA) glutaminase. KGA is mitochondrial specific protein whose expression in kidney is increased during metabolic acidosis. This process is mediated by an 8-base AU-sequence in KGA that functions as a pH-response element. The human KGA gene maps to chromosome 2, and produces three isoforms, designated KGA, GAC, and GAM, by alternative splicing. KGA is synthesized as a cytosolic protein that is transported to the mitochondria as an intermediate protein, and is further cleaved into the KGA isoform and the GAC isoform. The processing of the GAM isoform is unclear. The KGA isoform is abundant in brain and kidney, while the GAC isoform is principally expressed in cardiac muscle and pancreas. The GAM isoform is solely expressed in cardiac and skeletal muscle.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
化学信息
分子量Theoretical: 65 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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