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Anti-Glutaminase Antibody (8G424) 是一种 Rabbit 抗体,靶向 Glutaminase。Anti-Glutaminase Antibody (8G424) 可用于 WB,ICC/IF,IHC。
Anti-Glutaminase Antibody (8G424) 是一种 Rabbit 抗体,靶向 Glutaminase。Anti-Glutaminase Antibody (8G424) 可用于 WB,ICC/IF,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-Glutaminase Antibody (8G424) is a Rabbit antibody targeting Glutaminase. Anti-Glutaminase Antibody (8G424) can be used in WB,ICC/IF,IHC. |
别名 | L-glutamine amidohydrolase, KIAA0838, K-glutaminase, KGA, Glutaminase kidney isoform, mitochondrial, GLSK, GLS, GDPAG, GAM, GAC, EIEE71, EC:3.5.1.2, DEE71, CASGID, AAD20 |
Ig Type | IgG |
克隆号 | 8G424 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Glutaminase on different lysates using anti-Glutaminase antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutaminase antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutaminase antibody. Counter stained with hematoxylin. 4. ICC staining Glutaminase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Glutaminase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Glutamine is an important molecule involved in several cellular functions, including nitrogen and carbon transport, hepatic urea synthesis, renal ammoniagenesis, and gluconeogenesis. Glutamine is catabolized by either the liver-type (LGA) or kidney-type (KGA) glutaminase. KGA is mitochondrial specific protein whose expression in kidney is increased during metabolic acidosis. This process is mediated by an 8-base AU-sequence in KGA that functions as a pH-response element. The human KGA gene maps to chromosome 2, and produces three isoforms, designated KGA, GAC, and GAM, by alternative splicing. KGA is synthesized as a cytosolic protein that is transported to the mitochondria as an intermediate protein, and is further cleaved into the KGA isoform and the GAC isoform. The processing of the GAM isoform is unclear. The KGA isoform is abundant in brain and kidney, while the GAC isoform is principally expressed in cardiac muscle and pancreas. The GAM isoform is solely expressed in cardiac and skeletal muscle. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 65 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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