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Anti-GART Antibody (9K216) 是一种 Rabbit 抗体,靶向 GART。Anti-GART Antibody (9K216) 可用于 WB,ICC/IF,IHC,IP。
Anti-GART Antibody (9K216) 是一种 Rabbit 抗体,靶向 GART。Anti-GART Antibody (9K216) 可用于 WB,ICC/IF,IHC,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-GART Antibody (9K216) is a Rabbit antibody targeting GART. Anti-GART Antibody (9K216) can be used in WB,ICC/IF,IHC,IP. |
别名 | Trifunctional purine biosynthetic protein adenosine 3, PUR2_HUMAN, PRGS, Phosphoribosylglycinamide synthetase, Phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase, Phosphoribosylglycinamide formyltransferase, EC 2.1.2.29, Phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase, Phosphoribosylglycinamide formyltransferase, Phosphoribosyl-aminoimidazole synthetase, PGFT, PAIS, MGC47764, Glycinamide ribonucleotide synthetase, GARTF, GART, GARS, GAR transformylase, AIRS, AIR synthase, 5'-phosphoribosylglycinamide transformylase |
Ig Type | IgG |
克隆号 | 9K216 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of GART on different lysates using anti-GART antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293T, Lane 3: A431. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GART antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-GART antibody. Counter stained with hematoxylin. 4. ICC staining GART in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining GART in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining GART in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Purines are critical for energy metabolism, cell signaling and cell reproduction and also function as precursors for coenzymes, energy transfer molecules, regulatory factors and proteins involved in RNA and DNA synthesis. GART (GAR transformylase), also referred to as AIRS, GARS, PAIS, PGFT, PRGS or GARTF, is 1,010 amino acids in length and is a key folate-dependent trifunctional enzyme with phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and AICAR (phosphoribosylaminoimidazole synthetase) activity required for de novo purine biosynthesis. Cancer cells require considerable amounts of purines to sustain their accelerated growth and GART is, therefore, a target for cancer chemotherapy. GART is highly conserved in vertebrates. Two isoforms of GART are expressed due to alternative splicing events. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 107 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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