Anti-GAPDH Antibody (2N589) is a Mouse antibody targeting GAPDH. Anti-GAPDH Antibody (2N589) can be used in WB,ICC.

Human,Mouse,Rat,zebrafish

1. Western blot analysis of GAPDH on different cells lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, Lane 2: A549 cell lysate, Lane 3: HepG2 cell lysate, Lane 4: PC-12 cell lysate, Lane 5: F9 cell lysate.
2. ICC staining GAPDH in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
3. ICC staining of GAPDH in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor 555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

WB: 1:2000-5000; ICC: 1:200

Monoclonal

Unconjugated

Peptide