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Anti-FIS1 Antibody (9Z765)

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产品编号 TMAH-00438
别名 TTC11, TPR Repeat Protein 11, Tetratricopeptide Repeat Protein 11, Mitochondrial Fission 1 Protein, hFis1, FIS1 Homolog, FIS1, CGI-135

Anti-FIS1 Antibody (9Z765) 是一种抗体,靶向 FIS1。Anti-FIS1 Antibody (9Z765) 可用于 ELISA, WB, IHC。

Anti-FIS1 Antibody (9Z765)

Anti-FIS1 Antibody (9Z765)

Rating icon 还可以
产品编号 TMAH-00438 别名 TTC11, TPR Repeat Protein 11, Tetratricopeptide Repeat Protein 11, Mitochondrial Fission 1 Protein, hFis1, FIS1 Homolog, FIS1, CGI-135

Anti-FIS1 Antibody (9Z765) 是一种抗体,靶向 FIS1。Anti-FIS1 Antibody (9Z765) 可用于 ELISA, WB, IHC。

规格价格库存数量
50 μL
¥ 1,315
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100 μL
¥ 2,180
5日内发货
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产品介绍

生物活性
产品描述
Anti-FIS1 Antibody (9Z765) is an antibody targeting FIS1. Anti-FIS1 Antibody (9Z765) can be used in ELISA, WB, IHC.
别名TTC11, TPR Repeat Protein 11, Tetratricopeptide Repeat Protein 11, Mitochondrial Fission 1 Protein, hFis1, FIS1 Homolog, FIS1, CGI-135
Ig Type
Rabbit IgG
克隆号
9Z765
交叉反应
Human
验证活性
1. Western Blot
-Positive WB detected in: HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, U251 whole cell lysate, A549 whole cell lysate
-All lanes: FIS1 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 17 kDa
-Observed band size: 17-22 kDa
2. IHC image of TMAH-00438 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00438 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
应用
ELISA, WB, IHC
推荐剂量
WB:1:500-1:2000; IHC:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Mitochondrion outer membrane; Single-pass membrane protein. Peroxisome membrane; Single-pass membrane protein.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human FIS1
抗原种属
Human
基因ID
51024
Uniprot ID
研究领域
Cancer, Cell biology, Metabolism
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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