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Anti-FIS1 Antibody (9Z765) 是一种抗体,靶向 FIS1。Anti-FIS1 Antibody (9Z765) 可用于 ELISA, WB, IHC。
Anti-FIS1 Antibody (9Z765) 是一种抗体,靶向 FIS1。Anti-FIS1 Antibody (9Z765) 可用于 ELISA, WB, IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,315 | 5日内发货 | |
100 μL | ¥ 2,180 | 5日内发货 |
产品描述 | Anti-FIS1 Antibody (9Z765) is an antibody targeting FIS1. Anti-FIS1 Antibody (9Z765) can be used in ELISA, WB, IHC. |
别名 | TTC11, TPR Repeat Protein 11, Tetratricopeptide Repeat Protein 11, Mitochondrial Fission 1 Protein, hFis1, FIS1 Homolog, FIS1, CGI-135 |
Ig Type | Rabbit IgG |
克隆号 | 9Z765 |
交叉反应 | Human |
验证活性 | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, U251 whole cell lysate, A549 whole cell lysate -All lanes: FIS1 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 17 kDa -Observed band size: 17-22 kDa 2. IHC image of TMAH-00438 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00438 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
应用 | ELISA, WB, IHC |
推荐剂量 | WB:1:500-1:2000; IHC:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Mitochondrion outer membrane; Single-pass membrane protein. Peroxisome membrane; Single-pass membrane protein. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human FIS1 |
抗原种属 | Human |
基因ID | 51024 |
Uniprot ID | |
研究领域 | Cancer, Cell biology, Metabolism |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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