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Anti-Ephrin B2/EFNB2 Antibody (6Q379)
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产品编号 TMAB-00626
Anti-Ephrin B2/EFNB2 Antibody (6Q379) 是一种 Rabbit 抗体，靶向 Ephrin B2/EFNB2。Anti-Ephrin B2/EFNB2 Antibody (6Q379) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Ephrin B2/EFNB2 Antibody (6Q379)
Anti-Ephrin B2/EFNB2 Antibody (6Q379)
还可以产品编号 TMAB-00626
Anti-Ephrin B2/EFNB2 Antibody (6Q379) 是一种 Rabbit 抗体，靶向 Ephrin B2/EFNB2。Anti-Ephrin B2/EFNB2 Antibody (6Q379) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,395 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-Ephrin B2/EFNB2 Antibody (6Q379) is a Rabbit antibody targeting Ephrin B2/EFNB2. Anti-Ephrin B2/EFNB2 Antibody (6Q379) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 6Q379 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (TMAB-00626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (TMAB-00626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (TMAB-00626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (TMAB-00626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Rat) Lysate at 40 μg Lane 3: U251 (Human) Cell Lysate at 30 μg Primary: Anti-Ephrin B2 (TMAB-00626) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45-50 kDa Observed band size: 47 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Membrane. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008] |
| 免疫原 | KLH conjugated synthetic peptide: human Ephrin B2 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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