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Anti-eNOS Antibody (6A162)
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产品编号 TMAB-00615
Anti-eNOS Antibody (6A162) 是一种 Mouse 抗体，靶向 eNOS。Anti-eNOS Antibody (6A162) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-eNOS Antibody (6A162)
Anti-eNOS Antibody (6A162)
还可以产品编号 TMAB-00615
Anti-eNOS Antibody (6A162) 是一种 Mouse 抗体，靶向 eNOS。Anti-eNOS Antibody (6A162) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-eNOS Antibody (6A162) is a Mouse antibody targeting eNOS. Anti-eNOS Antibody (6A162) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 6A162 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Sample: Liver (mouse) Lysate at 40 μg Primary: Anti-eNOS (TMAB-00615) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 133 kDa Observed band size: 135 kDa 2. Sample: Cerebrum (Mouse) Lysate at 40 μg Cerebrum (Rat) Lysate at 40 μg Lung (Mouse) Lysate at 40 μg Placenta (Mouse) Lysate at 40 μg Primary: Anti-eNOS (TMAB-00615) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 135 kDa Observed band size: 135 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (eNOS/NOS-3) Monoclonal Antibody, Unconjugated (TMAB-00615) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). |
| 免疫原 | KLH conjugated synthetic peptide: human eNos |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Response to hypoxia,Platelets,Nitric oxide associated,Oxidative Stress,Hypoxia,Oxidative stress,NOS |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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