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Anti-Cyclin B2 Antibody (9U494)
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产品编号 TMAC-01035
别名 MGC140694, MGC108931, HsT17299, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, G2 mitotic specific cyclin B2, Cyclin B2, CycB2, CCNB2_HUMAN, ccnb2
Anti-Cyclin B2 Antibody (9U494) 是一种 Rabbit 抗体，靶向 Cyclin B2。Anti-Cyclin B2 Antibody (9U494) 可用于 WB,ICC/IF,IHC,IP。
Anti-Cyclin B2 Antibody (9U494)
Anti-Cyclin B2 Antibody (9U494)
还可以产品编号 TMAC-01035 别名 MGC140694, MGC108931, HsT17299, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, G2 mitotic specific cyclin B2, Cyclin B2, CycB2, CCNB2_HUMAN, ccnb2
Anti-Cyclin B2 Antibody (9U494) 是一种 Rabbit 抗体，靶向 Cyclin B2。Anti-Cyclin B2 Antibody (9U494) 可用于 WB,ICC/IF,IHC,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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| 产品描述 | Anti-Cyclin B2 Antibody (9U494) is a Rabbit antibody targeting Cyclin B2. Anti-Cyclin B2 Antibody (9U494) can be used in WB,ICC/IF,IHC,IP. |
| 别名 | MGC140694, MGC108931, HsT17299, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, G2 mitotic specific cyclin B2, Cyclin B2, CycB2, CCNB2_HUMAN, ccnb2 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Cyclin B2 on different lysates using anti-Cyclin B2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: K562. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin. 4. ICC staining Cyclin B2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Cyclin B2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex . Evidence has established that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B-type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 45 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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