Anti-Complement Factor D/CFD Polyclonal Antibody is a Rabbit antibody targeting Complement Factor D/CFD. Anti-Complement Factor D/CFD Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Human,Mouse (predicted:Rat)

1. Protein:
A549 (human) cell lysates at 40 μg;
spleen disease in mouse at 40 μg;
Primary: Anti-Factor D (TMAB-00467) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (secondary antibody) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size:24 kDa
Observed band size:28/75 kDa
2. Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Factor D Polyclonal Antibody, Unconjugated (TMAB-00467) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Factor D/Adipsin

Human

Alternative Pathway