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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-CEA Antibody (1A582)

货号 TMAB-04197 一键复制产品信息
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Anti-CEA Antibody (1A582) 是一种 Mouse 抗体，靶向 CEA。Anti-CEA Antibody (1A582) 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-CEA Antibody (1A582)

一键复制产品信息
Rating icon 还可以
货号 TMAB-04197

Anti-CEA Antibody (1A582) 是一种 Mouse 抗体，靶向 CEA。Anti-CEA Antibody (1A582) 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-CEA Antibody (1A582)
规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-CEA Antibody (1A582) is a Mouse antibody targeting CEA. Anti-CEA Antibody (1A582) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
克隆号
1A582
反应种属
Human
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CEA) Monoclonal Antibody, Unconjugated (TMAB-04197) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Sample:
Lane 1: Human LOVO cell lysates
Lane 2: Recombinant human CEACAM5 protein, C-His
Primary: Anti-CEACAM5 (TMAB-04197) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 200, 140 kDa
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500
抗体种类
Monoclonal
宿主来源Mouse
亚细胞定位Cell membrane; Lipid-anchor, GPI-anchor
组织特异性Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
构建方式Hybridoma Monoclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
抗原信息
免疫原
KLH conjugated synthetic peptide: human CEA/CD66e/CEACAM5
抗原种属
Human
基因名称
CEACAM5
基因ID
1048
蛋白名称
Carcinoembryonic antigen-related cell adhesion molecule 6
Uniprot ID
P06731
功能
Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
化学信息
分子量Theoretical: 150-200 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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