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Anti-CDK4 Antibody (6X741)
还可以产品编号 TMAH-00253
别名 PSK-J3, cyclin-dependent kinase 4, CMM3
Anti-CDK4 Antibody (6X741) 是一种抗体，靶向 CDK4。Anti-CDK4 Antibody (6X741) 可用于 ELISA, WB, IP。
Anti-CDK4 Antibody (6X741)
Anti-CDK4 Antibody (6X741)
还可以产品编号 TMAH-00253 别名 PSK-J3, cyclin-dependent kinase 4, CMM3
Anti-CDK4 Antibody (6X741) 是一种抗体，靶向 CDK4。Anti-CDK4 Antibody (6X741) 可用于 ELISA, WB, IP。
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抗原信息
| 产品描述 | Anti-CDK4 Antibody (6X741) is an antibody targeting CDK4. Anti-CDK4 Antibody (6X741) can be used in ELISA, WB, IP. |
| 别名 | PSK-J3, cyclin-dependent kinase 4, CMM3 |
| Ig Type | Rabbit IgG |
| 克隆号 | 6X741 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate -All lanes: CDK4 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 34, 21 kDa -Observed band size: 34 kDa 2. Immunoprecipitating CDK4 in Hela whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00253 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500) -Lane 2:TMAH-00253(2µg)+ Hela whole cell lysate(500µg) -Lane 3: Hela whole cell lysate (10µg) |
| 应用 | ELISA, WB, IP |
| 推荐剂量 | WB:1:500-1:5000; IP:1:200-1:1000. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Nucleus. Nucleus membrane. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates S... |
| 偶联 | Unconjugated |
| 免疫原 | A synthesized peptide derived from human CDK4 |
| 抗原种属 | Human |
| 基因ID | 1019 |
| Uniprot ID | |
| 研究领域 | Isotype/Loading Controls, Epigenetics and Nuclear Signaling, Cancer, Cell biology |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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