Anti-CDC5L Antibody (9X496) is an antibody targeting CDC5L. Anti-CDC5L Antibody (9X496) can be used in ELISA, WB, IHC, IF, IP.

Rabbit IgG

9X496

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate
-All lanes: CDC5L antibody at 1.3μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 93 KDa
-Observed band size: 100 KDa
2. IHC image of TMAH-00242 diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
3. IHC image of TMAH-00242 diluted at 1:126.5 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4. Immunofluorescence staining of A549 cells with TMAH-00242 at 1:42, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
5. Immunoprecipitating CDC5L in Hela whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00242 in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00242 (3μg) + Hela whole cell lysate (500μg)
-Lane 3: Hela whole cell lysate (20μg)

WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200; IP:1:200-1:1000.

Monoclonal

Unconjugated

A synthetic peptide: Human CDC5L

Human

Epigenetics and Nuclear Signaling