Anti-CD81 Polyclonal Antibody is a Rabbit antibody targeting CD81. Anti-CD81 Polyclonal Antibody can be used in WB,FCM,ELISA.

IgG

Human (predicted:Mouse,Rat)

1. Sample:
Lane 1: Recombinant human CD81 protein, hFc (HEK293)
Primary: Anti-CD81 (TMAB-00395) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 36 kDa
2. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CD81 antibody (TMAB-00395)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 2ug/Test; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human TAPA1/CD81

Human

CD,Host Virus Interaction,Receptor