Anti-CD63 Antibody (7S689) is a Rabbit antibody targeting CD63. Anti-CD63 Antibody (7S689) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

7S689

Human

1. 25 μg total protein per Lane of various lysates probed with CD63 monoclonal antibody, unconjugated (TMAB-00378) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD63 Monoclonal Antibody, Unconjugated (TMAB-00378) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD63 Monoclonal Antibody, Unconjugated (TMAB-00378) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD63 Monoclonal Antibody, Unconjugated (TMAB-00378) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. 4% Paraformaldehyde-fixed A375 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (CD63) monoclonal Antibody, unconjugated (TMAB-00378) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500

Monoclonal

A synthesized peptide: human CD63

Human

CD63

CD63 antigen

Cell adhesion,Tumor antigens,Platelets,Platelets,Tumor Associated

This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.