Anti-CD46 Antibody (1X173) is an antibody targeting CD46. Anti-CD46 Antibody (1X173) can be used in ELISA, WB, IHC.

Rabbit IgG

1X173

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate
-All lanes: CD46 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 44, 45, 43, 41, 42, 40, 37 kDa
-Observed band size: 56 kDa
2. IHC image of TMAH-00204 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00204 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

ELISA, WB, IHC

WB:1:500-1:5000; IHC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human CD46

Human

4179

Immunology