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Anti-CCDC134 Antibody (8H634) 是一种 Rabbit 抗体,靶向 CCDC134。Anti-CCDC134 Antibody (8H634) 可用于 WB,IP。
Anti-CCDC134 Antibody (8H634) 是一种 Rabbit 抗体,靶向 CCDC134。Anti-CCDC134 Antibody (8H634) 可用于 WB,IP。
产品描述 | Anti-CCDC134 Antibody (8H634) is a Rabbit antibody targeting CCDC134. Anti-CCDC134 Antibody (8H634) can be used in WB,IP. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 8H634 |
交叉反应 | Human |
验证活性 | 1. Anti-CCDC134 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HL-60 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:26 kDa. 2. CCDC134 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-CCDC134 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CCDC134 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 28 kDa. -Observed band size: 28 kDa |
应用 | WB,IP |
推荐剂量 | WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human CCDC134 (rh CCDC134; TMPY-02551; Q9H6E4; Met1-Leu229). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | Coiled-coil domain containing 134 (CCDC134) is a 229 amino acids secretory protein. Coiled-coil domain is a motif in which alpha-helix are coiled together. It has been found in many types of proteins, including transcription factors, intermediate filaments and certain tRNA synthetases. Many proteins containing such motif CCDC134 are involved in important biological functions. CCDC134 is also considered as a novel human MAPK-regulating protein that can inhibit the MAPK pathway. This protein significantly inhibite Elk1 transcriptional activity. The coiled-coil domain is a ubiquitous protein motif that is often involved in oligomerization. |
偶联 | Unconjugated |
免疫原 | Recombinant Human CCDC134 protein (TMPY-02551) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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