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Anti-CCDC134 Antibody (8H634)

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产品编号 TMAY-02519

Anti-CCDC134 Antibody (8H634) 是一种 Rabbit 抗体,靶向 CCDC134。Anti-CCDC134 Antibody (8H634) 可用于 WB,IP。

Anti-CCDC134 Antibody (8H634)

Anti-CCDC134 Antibody (8H634)

Rating icon 还可以
产品编号 TMAY-02519

Anti-CCDC134 Antibody (8H634) 是一种 Rabbit 抗体,靶向 CCDC134。Anti-CCDC134 Antibody (8H634) 可用于 WB,IP。

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产品介绍

生物活性
产品描述
Anti-CCDC134 Antibody (8H634) is a Rabbit antibody targeting CCDC134. Anti-CCDC134 Antibody (8H634) can be used in WB,IP.
Ig Type
Monoclonal Rabbit IgG
克隆号
8H634
交叉反应
Human
验证活性
1. Anti-CCDC134 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HL-60 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:26 kDa.

-Observed band size:26 kDa.

2. CCDC134 was immunoprecipitated using:

-Lane A:0.5 mg HepG2 Whole Cell Lysate.

-2 µL anti-CCDC134 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-CCDC134 rabbit monoclonal antibody, at 1:200 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 28 kDa.

-Observed band size: 28 kDa
应用
WB,IP
推荐剂量
WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human CCDC134 (rh CCDC134; TMPY-02551; Q9H6E4; Met1-Leu229).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Coiled-coil domain containing 134 (CCDC134) is a 229 amino acids secretory protein. Coiled-coil domain is a motif in which alpha-helix are coiled together. It has been found in many types of proteins, including transcription factors, intermediate filaments and certain tRNA synthetases. Many proteins containing such motif CCDC134 are involved in important biological functions. CCDC134 is also considered as a novel human MAPK-regulating protein that can inhibit the MAPK pathway. This protein significantly inhibite Elk1 transcriptional activity. The coiled-coil domain is a ubiquitous protein motif that is often involved in oligomerization.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Human CCDC134 protein (TMPY-02551)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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