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Anti-Carbonic Anhydrase 14 Antibody (5R147)
还可以产品编号 TMAY-00855
Anti-Carbonic Anhydrase 14 Antibody (5R147) 是一种 Rabbit 抗体，靶向 Carbonic Anhydrase 14。Anti-Carbonic Anhydrase 14 Antibody (5R147) 可用于 ELISA,IHC-P,ICC/IF。
Anti-Carbonic Anhydrase 14 Antibody (5R147)
Anti-Carbonic Anhydrase 14 Antibody (5R147)
还可以产品编号 TMAY-00855
Anti-Carbonic Anhydrase 14 Antibody (5R147) 是一种 Rabbit 抗体，靶向 Carbonic Anhydrase 14。Anti-Carbonic Anhydrase 14 Antibody (5R147) 可用于 ELISA,IHC-P,ICC/IF。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-Carbonic Anhydrase 14 Antibody (5R147) is a Rabbit antibody targeting Carbonic Anhydrase 14. Anti-Carbonic Anhydrase 14 Antibody (5R147) can be used in ELISA,IHC-P,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 5R147 |
| 交叉反应 | Human; Not React with:Mouse, Rat |
| 验证活性 | 1. Immunofluorescence staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane. 2. Immunochemical staining of human CA14 in human liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of hepatocyte. |
| 应用 | ELISA,IHC-P,ICC/IF |
| 推荐剂量 | ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase XIV / CA14 (rh Carbonic Anhydrase XIV / CA14; TMPY-00967; NP_036245.1; Met 1-Met 290). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Carbonic Anhydrase XIV / CA14 Protein (TMPY-00967) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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