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Anti-Carbonic Anhydrase 12 Antibody (3Q864)
还可以产品编号 TMAY-01030
Anti-Carbonic Anhydrase 12 Antibody (3Q864) 是一种 Mouse 抗体，靶向 Carbonic Anhydrase 12。Anti-Carbonic Anhydrase 12 Antibody (3Q864) 可用于 ICC/IF。
Anti-Carbonic Anhydrase 12 Antibody (3Q864)
Anti-Carbonic Anhydrase 12 Antibody (3Q864)
还可以产品编号 TMAY-01030
Anti-Carbonic Anhydrase 12 Antibody (3Q864) 是一种 Mouse 抗体，靶向 Carbonic Anhydrase 12。Anti-Carbonic Anhydrase 12 Antibody (3Q864) 可用于 ICC/IF。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-Carbonic Anhydrase 12 Antibody (3Q864) is a Mouse antibody targeting Carbonic Anhydrase 12. Anti-Carbonic Anhydrase 12 Antibody (3Q864) can be used in ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 3Q864 |
| 交叉反应 | Human |
| 验证活性 | Immunofluorescence staining of Human CA12 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CA12 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytomembrane. |
| 应用 | ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase XII / CA12 (rh Carbonic Anhydrase XII / CA12; TMPY-00694; NP_001209.1; Met1-Gln291). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| 研究背景 | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Carbonic Anhydrase XII / CA12 protein (TMPY-00694) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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