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Anti-BMP-4 Antibody (3R788)
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产品编号 TMAH-00117
别名 ZYME, OFC11, MCOPS6, bone morphogenetic protein 4, BMP2B1, BMP2B
Anti-BMP-4 Antibody (3R788) 是一种抗体，靶向 BMP-4。Anti-BMP-4 Antibody (3R788) 可用于 ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200。
Anti-BMP-4 Antibody (3R788)
Anti-BMP-4 Antibody (3R788)
还可以产品编号 TMAH-00117 别名 ZYME, OFC11, MCOPS6, bone morphogenetic protein 4, BMP2B1, BMP2B
Anti-BMP-4 Antibody (3R788) 是一种抗体，靶向 BMP-4。Anti-BMP-4 Antibody (3R788) 可用于 ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
生物活性
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抗原信息
| 产品描述 | Anti-BMP-4 Antibody (3R788) is an antibody targeting BMP-4. Anti-BMP-4 Antibody (3R788) can be used in ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200. |
| 别名 | ZYME, OFC11, MCOPS6, bone morphogenetic protein 4, BMP2B1, BMP2B |
| Ig Type | Rabbit IgG |
| 克隆号 | 3R788 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: Hela whole cell lysate -All lanes: BMP4 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 47 kDa -Observed band size: 36-55 kDa 2. Immunofluorescence staining of Hela cell with TMAH-00117 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 508-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| 应用 | |
| 推荐剂量 | WB:1:500-1:2000; IF:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Secreted, extracellular space, extracellular matrix. |
| 组织特异性 | Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical BMP pathways such as ERK/MAP kinase, PI3K/Akt, or SRC cascades. For example, induces SRC phosphorylation which, in turn, activates VEGFR2, leading to an angiogenic response. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human BMP4 |
| 抗原种属 | Human |
| 基因名称 | BMP4 |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cancer, Cardiovascular, Developmental biology, Signal transduction, Stem cells |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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