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Anti-Bim Antibody (2H443)
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产品编号 TMAC-00441
别名 BOD, BIM, BCL2-like 11 (apoptosis facilitator), BAM
Anti-Bim Antibody (2H443) 是一种 Rabbit 抗体，靶向 Bim。Anti-Bim Antibody (2H443) 可用于 WB,ICC,IHC,IP。
Anti-Bim Antibody (2H443)
Anti-Bim Antibody (2H443)
还可以产品编号 TMAC-00441 别名 BOD, BIM, BCL2-like 11 (apoptosis facilitator), BAM
Anti-Bim Antibody (2H443) 是一种 Rabbit 抗体，靶向 Bim。Anti-Bim Antibody (2H443) 可用于 WB,ICC,IHC,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-Bim Antibody (2H443) is a Rabbit antibody targeting Bim. Anti-Bim Antibody (2H443) can be used in WB,ICC,IHC,IP. |
| 别名 | BOD, BIM, BCL2-like 11 (apoptosis facilitator), BAM |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of Bim on different lysates using anti-Bim antibody at 1/1,000 dilution. Positive control: Lane 1: Raji, Lane 2: Jurkat, Lane 3: A431. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Bim antibody. Counter stained with hematoxylin. 3. ICC staining Bim in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Bim in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Bim in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Pro-apototic Bcl-2 family members promote cell death by neutralizing their apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 22/16/13 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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