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Anti-BCL2 Antibody (6L780)
还可以产品编号 TMAY-00433
Anti-BCL2 Antibody (6L780) 是一种 Mouse 抗体，靶向 BCL2。Anti-BCL2 Antibody (6L780) 可用于 WB,IP。
Anti-BCL2 Antibody (6L780)
Anti-BCL2 Antibody (6L780)
还可以产品编号 TMAY-00433
Anti-BCL2 Antibody (6L780) 是一种 Mouse 抗体，靶向 BCL2。Anti-BCL2 Antibody (6L780) 可用于 WB,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-BCL2 Antibody (6L780) is a Mouse antibody targeting BCL2. Anti-BCL2 Antibody (6L780) can be used in WB,IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 6L780 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-BCL2 mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: U937 Whole Cell Lysate. -Lane D: HL-60 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:27 kDa. 2. BCL2 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg K562 Whole Cell Lysate. -2 µL anti-BCL2 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-BCL2 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 20 kDa. -Observed band size: 20 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BCL2 / Bcl-2 (rh BCL2 / Bcl-2; TMPY-02700; P10415-1; Met1-Asp211). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human BCL2 / Bcl-2 protein (TMPY-02700) |
| 抗原种属 | Human |
| 研究领域 | Cancer Drug Targets |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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