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Anti-BCL2 Antibody (3D343) 是一种抗体,靶向 BCL2。Anti-BCL2 Antibody (3D343) 可用于 ELISA, WB, IHC。
Anti-BCL2 Antibody (3D343) 是一种抗体,靶向 BCL2。Anti-BCL2 Antibody (3D343) 可用于 ELISA, WB, IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,305 | 5日内发货 | |
100 μL | ¥ 2,190 | 5日内发货 |
产品描述 | Anti-BCL2 Antibody (3D343) is an antibody targeting BCL2. Anti-BCL2 Antibody (3D343) can be used in ELISA, WB, IHC. |
别名 | PPP1R50, Bcl-2, B-cell CLL/lymphoma 2 |
Ig Type | Rabbit IgG |
克隆号 | 3D343 |
交叉反应 | Human, Mouse |
验证活性 | 1. Western Blot -Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U-937 whole cell lysate, THP-1 whole cell lysate, Raji whole cell lysate, Mouse Spleen whole cell lysate -All lanes: Bcl-2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 27, 23 kDa -Observed band size: 27 kDa 2. IHC image of TMAH-00109 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
应用 | ELISA, WB, IHC |
推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human Bcl-2 |
抗原种属 | Human |
基因ID | 596 |
Uniprot ID | |
研究领域 | Cancer, Cell biology, Metabolism, Signal transduction |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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