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Anti-BCL-XL Antibody (2A140)

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产品编号 TMAH-00111
别名 PPP1R52, bcl-xS, BCLXS, BCL-XL/S, bcl-xL, BCLXL, Bcl-X, BCLX, BCL2-like 1, BCL2L

Anti-BCL-XL Antibody (2A140) 是一种抗体,靶向 BCL-XL。Anti-BCL-XL Antibody (2A140) 可用于 ELISA, WB, IHC。

Anti-BCL-XL Antibody (2A140)

Anti-BCL-XL Antibody (2A140)

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产品编号 TMAH-00111 别名 PPP1R52, bcl-xS, BCLXS, BCL-XL/S, bcl-xL, BCLXL, Bcl-X, BCLX, BCL2-like 1, BCL2L

Anti-BCL-XL Antibody (2A140) 是一种抗体,靶向 BCL-XL。Anti-BCL-XL Antibody (2A140) 可用于 ELISA, WB, IHC。

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产品介绍

生物活性
产品描述
Anti-BCL-XL Antibody (2A140) is an antibody targeting BCL-XL. Anti-BCL-XL Antibody (2A140) can be used in ELISA, WB, IHC.
别名PPP1R52, bcl-xS, BCLXS, BCL-XL/S, bcl-xL, BCLXL, Bcl-X, BCLX, BCL2-like 1, BCL2L
Ig Type
Rabbit IgG
克隆号
2A140
交叉反应
Human, Mouse, Rat
验证活性
1. Western Blot
-Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate
-All lanes: Bcl-XL antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 27, 19, 26 kDa
-Observed band size: 30 kDa
2. IHC image of TMAH-00111 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
应用
ELISA, WB, IHC
推荐剂量
WB:1:500-1:5000; IHC:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位[Isoform Bcl-X(L)]: Mitochondrion inner membrane. Mitochondrion outer membrane. Mitochondrion matrix. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.; Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP av...
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthesized peptide derived from human Bcl-XL
抗原种属
Human
基因ID
598
Uniprot ID
研究领域
Cancer, Cell biology, Metabolism, Signal transduction
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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