Anti-AXL Antibody (2V194)

Name: Anti-AXL Antibody (2V194)
Brand: TargetMol
SKU: TMAH-00099
Rating: 4.5 (1 reviews)

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产品编号 TMAH-00099

别名 UFO, Tyro7, JTK11, EC 2.7.10.1, EC 2.7.10, AXL oncogene, Axl, ARK, AI323647

Anti-AXL Antibody (2V194) 是一种抗体，靶向 AXL。Anti-AXL Antibody (2V194) 可用于 ELISA。

Anti-AXL Antibody (2V194)

还可以

产品编号 TMAH-00099 别名 UFO, Tyro7, JTK11, EC 2.7.10.1, EC 2.7.10, AXL oncogene, Axl, ARK, AI323647

Anti-AXL Antibody (2V194) 是一种抗体，靶向 AXL。Anti-AXL Antibody (2V194) 可用于 ELISA。

规格	价格	库存	数量
50 μL	¥ 1,310	5日内发货
100 μL	¥ 2,190	5日内发货

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生物活性

产品描述	Anti-AXL Antibody (2V194) is an antibody targeting AXL. Anti-AXL Antibody (2V194) can be used in ELISA.
别名	UFO, Tyro7, JTK11, EC 2.7.10.1, EC 2.7.10, AXL oncogene, Axl, ARK, AI323647
Ig Type	hIgG1
克隆号	2V194
交叉反应	Human
验证活性	1. The Binding Activity of Human AXL with Anti-AXL recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized human AXL at 2 μg/mL can bind Anti-AXL recombinant antibody. The EC50 is 1.308-1.500 ng/mL. 2. Immunofluorescence staining of Hela cell with TMAH-00099 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L). 3. Immunofluorescence staining of PC-3 cell with TMAH-00099 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L). 4. The purity of AXL was greater than 90% as determined by SEC-HPLC 5. 786-O cells were stained with Human IgG1&Igκ Isotype Control (green line) and anti-AXL antibody (TMAH-00099) (2µg/1*10^6 cells) (red line), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
应用	ELISA
推荐剂量	ELISA:1:5000-1:50000.
抗体种类	Monoclonal
亚细胞定位	Cell membrane; Single-pass type I membrane protein.
构建方式	Recombinant Antibody
纯化方式	Affinity-chromatography
性状	Liquid
缓冲液	Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
研究背景	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope. (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	Recombinant Protein: Human AXL Protein
抗原种属	Human
基因ID	558
Uniprot ID	P30530
研究领域	Cancer, Cell biology, Tags & Cell Markers, Signal transduction