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Anti-AURKA Antibody (1Y815) 是一种 Rabbit 抗体,靶向 AURKA。Anti-AURKA Antibody (1Y815) 可用于 WB,ICC/IF,FCM。
Anti-AURKA Antibody (1Y815) 是一种 Rabbit 抗体,靶向 AURKA。Anti-AURKA Antibody (1Y815) 可用于 WB,ICC/IF,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-AURKA Antibody (1Y815) is a Rabbit antibody targeting AURKA. Anti-AURKA Antibody (1Y815) can be used in WB,ICC/IF,FCM. |
别名 | AYK 1 |
Ig Type | IgG |
交叉反应 | Human,Mouse |
验证活性 | 1. ICC staining Aurora A in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 2. ICC staining Aurora A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Aurora A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of Hela cells with Aurora A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-2000; ICC/IF: 1:100-500; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Activation of the oncogenic protein kinase Aurora A regulates meiotic and mitotic cell cycles in eukaryotic cells. Specifically, Aurora A plays a key role in G2/M progression. Activation occurs via autophosphorylation, and while 14 sites are subject to this, only the threonine residue at position 295 is required for activity. Though autophosphorylation mediates activation, a number of other proteins influence activation, including the spindle assembly factor TPX2 and p53 |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 46 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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