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Anti-ASH2L Antibody (4J278)
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产品编号 TMAC-00282
别名 Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, ASH2L1, ASH2L
Anti-ASH2L Antibody (4J278) 是一种 Rabbit 抗体，靶向 ASH2L。Anti-ASH2L Antibody (4J278) 可用于 WB,ICC/IF,IHC。
Anti-ASH2L Antibody (4J278)
Anti-ASH2L Antibody (4J278)
还可以产品编号 TMAC-00282 别名 Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, ASH2L1, ASH2L
Anti-ASH2L Antibody (4J278) 是一种 Rabbit 抗体，靶向 ASH2L。Anti-ASH2L Antibody (4J278) 可用于 WB,ICC/IF,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
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| 产品描述 | Anti-ASH2L Antibody (4J278) is a Rabbit antibody targeting ASH2L. Anti-ASH2L Antibody (4J278) can be used in WB,ICC/IF,IHC. |
| 别名 | Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, ASH2L1, ASH2L |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of ASH2L on different lysates using anti-ASH2L antibody at 1/1,000 dilution. Positive control: Lane 1: PC12, Lane 2: 293T. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASH2L antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ASH2L antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-ASH2L antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ASH2L antibody. Counter stained with hematoxylin. 6. ICC staining ASH2L in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining ASH2L in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining ASH2L in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:200-500; ICC/IF: 1:200-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1, or isoform 1, which contains a nuclear localization signal and PHD finger motif, suggesting that the gene product functions as a transcription regulator. Alternative splicing results in a shorter isoform 2, designated ASH2L2, which is missing the first 94 amino acid residues found in ASH2L1. Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates expression of certain genes in early development, and contain similar, but not identical, domains, including a zinc finger motif. ASH2L is highly expressed in fetal liver, testis and leukemia cell lines with erythroid and megakaryocytic potential, such as K562, Hel and Dami. Differentiation inducers (e.g. phorbol ester and hemin) cause different expression patterns in these cells lines, suggesting that ASH2L plays a role in hematopoiesis and is associated with particular types of leukemia. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 69 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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