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Anti-Apolipoprotein A-I/APOA1 Antibody (4W607)
还可以产品编号 TMAY-01058
Anti-Apolipoprotein A-I/APOA1 Antibody (4W607) 是一种 Mouse 抗体，靶向 Apolipoprotein A-I/APOA1。Anti-Apolipoprotein A-I/APOA1 Antibody (4W607) 可用于 ICC/IF。
Anti-Apolipoprotein A-I/APOA1 Antibody (4W607)
Anti-Apolipoprotein A-I/APOA1 Antibody (4W607)
还可以产品编号 TMAY-01058
Anti-Apolipoprotein A-I/APOA1 Antibody (4W607) 是一种 Mouse 抗体，靶向 Apolipoprotein A-I/APOA1。Anti-Apolipoprotein A-I/APOA1 Antibody (4W607) 可用于 ICC/IF。
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偶联与修饰
抗原信息
| 产品描述 | Anti-Apolipoprotein A-I/APOA1 Antibody (4W607) is a Mouse antibody targeting Apolipoprotein A-I/APOA1. Anti-Apolipoprotein A-I/APOA1 Antibody (4W607) can be used in ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 4W607 |
| 交叉反应 | Human |
| 验证活性 | Immunofluorescence staining of APOA1 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human APOA1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). |
| 应用 | ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Apolipoprotein A-I/APOA1 (rh Apolipoprotein A-I/APOA1; TMPY-03428; P02647; Asp25-Gln267). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Apolipoprotein A-I/APOA1 Protein (TMPY-03428) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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