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Anti-ANXA2 Antibody (7P81) 是一种抗体,靶向 ANXA2。Anti-ANXA2 Antibody (7P81) 可用于 ELISA, WB, IHC。
Anti-ANXA2 Antibody (7P81) 是一种抗体,靶向 ANXA2。Anti-ANXA2 Antibody (7P81) 可用于 ELISA, WB, IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,310 | 5日内发货 | |
100 μL | ¥ 2,180 | 5日内发货 |
产品描述 | Anti-ANXA2 Antibody (7P81) is an antibody targeting ANXA2. Anti-ANXA2 Antibody (7P81) can be used in ELISA, WB, IHC. |
别名 | PAP-IV, p36, LPC2DP36, LPC2D, Lipocortin II, LIP2PAP-IV, Chromobindin-8, CAL1H, ANXA2, ANX2L4, ANX2, Annexin II, Annexin A2 |
Ig Type | Rabbit IgG |
克隆号 | 7P81 |
交叉反应 | Human |
验证活性 | 1. Western Blot -Positive WB detected in: HEK293 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate -All lanes: ANXA2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 39, 41 kDa -Observed band size: 36-45 kDa 2. IHC image of TMAH-00065 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
应用 | |
推荐剂量 | WB:1:500-1:2000; IHC:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Secreted, extracellular space, extracellular matrix, basement membrane. Melanosome. Note=In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human ANXA2 |
抗原种属 | Human |
基因名称 | ANXA2 |
基因ID | |
Uniprot ID | |
研究领域 | Signal transduction |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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