Anti-ANGPT2/Angiopoietin-2 Antibody (6U152) is an antibody targeting ANGPT2/Angiopoietin-2. Anti-ANGPT2/Angiopoietin-2 Antibody (6U152) can be used in ELISA, IHC.

Rabbit IgG

6U152

Human

1. IHC image of TMAH-00059 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
2. IHC image of TMAH-00059 diluted at 1:50 and staining in paraffin-embedded human Liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
3. Western Blot
-Positive WB detected in: Hela whole cell lysate(30µg), K562 whole cell lysate(30µg), U251 whole cell lysate(30µg),Mouse Heart tissue lysate(30µg),Mouse Brain tissue lysate(30µg),Rat Heart tissue lysate(30µg),Rat Brain tissue lysate(30µg)
-All lanes: ANGPT2 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/20000 dilution
-Predicted band size: 57 kDa
-Observed band size: 57 kDa
-Exposure time: 120s
4. IHC image of TMAH-00059 diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

ELISA, IHC

IHC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human Angiopoietin 2

Human

285

Cardiovascular