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Anti-Aminopeptidase N/CD13 Antibody (9N42)
还可以产品编号 TMAY-00181
Anti-Aminopeptidase N/CD13 Antibody (9N42) 是一种 Mouse 抗体，靶向 Aminopeptidase N/CD13。Anti-Aminopeptidase N/CD13 Antibody (9N42) 可用于 WB,IHC-P。
Anti-Aminopeptidase N/CD13 Antibody (9N42)
Anti-Aminopeptidase N/CD13 Antibody (9N42)
还可以产品编号 TMAY-00181
Anti-Aminopeptidase N/CD13 Antibody (9N42) 是一种 Mouse 抗体，靶向 Aminopeptidase N/CD13。Anti-Aminopeptidase N/CD13 Antibody (9N42) 可用于 WB,IHC-P。
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抗原信息
| 产品描述 | Anti-Aminopeptidase N/CD13 Antibody (9N42) is a Mouse antibody targeting Aminopeptidase N/CD13. Anti-Aminopeptidase N/CD13 Antibody (9N42) can be used in WB,IHC-P. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 9N42 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-ANPEP mouse monoclonal antibody at 1:500 dilution. -Lane A: THP-1 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:110 kDa. -Observed band size:140 kDa. 2. Immunochemical staining of human ANPEP in human kidney with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. |
| 应用 | WB,IHC-P |
| 推荐剂量 | WB: 1:500-1:2000; IHC-P: 1:100-1:500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD13/ANPEP (rh CD13/ANPEP; TMPY-00665; NP_001141.2; Lys69-Lys967,603Met/Ile). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human CD13/ANPEP Protein (TMPY-00665) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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