Anti-Alpha-fetoprotein Antibody (5G216) is a Mouse antibody targeting Alpha-fetoprotein. Anti-Alpha-fetoprotein Antibody (5G216) can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

5G216

Human,Mouse,Rat

1. Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human AFP Antibody, the EC50 is 24.94 ng/mL.
2. 25 μg total protein per Lane of various lysates probed with AFp (A2) monoclonal antibody, unconjugated (TMAB-00104) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
3. (Negative control) paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFp (A2) Monoclonal Antibody, Unconjugated (TMAB-00104) at 1:500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. (Negative control) paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFp (A2) Monoclonal Antibody, Unconjugated (TMAB-00104) at 1:500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFp (A2) Monoclonal Antibody, Unconjugated (TMAB-00104) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Monoclonal

AFP protein

Tumor antigens,Serum Proteins,Liver development,Cardiovascular ELISA kits