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Anti-AGTR1 Polyclonal Antibody 2
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产品编号 TMAB-00079
Anti-AGTR1 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 AGTR1。Anti-AGTR1 Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-AGTR1 Polyclonal Antibody 2
Anti-AGTR1 Polyclonal Antibody 2
还可以产品编号 TMAB-00079
Anti-AGTR1 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 AGTR1。Anti-AGTR1 Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-AGTR1 Polyclonal Antibody 2 is a Rabbit antibody targeting AGTR1. Anti-AGTR1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (TMAB-00079) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Liver (Mouse) Lysate at 40 μg Lane 2: Heart (Mouse) Lysate at 40 μg Lane 3: Placenta (Mouse) Lysate at 40 μg Lane 4: Kidney (Mouse) Lysate at 40 μg Lane 5: Embryo (Mouse) Lysate at 40 μg Lane 6: Cerebrum (Mouse) Lysate at 40 μg Lane 7: NIH/3T3 (Mouse) cell Lysate at 30 μg Lane 8: Liver (Rat) Lysate at 40 μg Lane 9: Heart (Rat) Lysate at 40 μg Lane 10: Kidney (Rat) Lysate at 40 μg Lane 11: Cerebrum (Rat) Lysate at 40 μg Lane 12: Embryo (Rat) Lysate at 40 μg Primary: Anti-Angiotensin II type 1A receptor (TMAB-00079) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 46 kDa 3. Sample: A549 (Human) Cell Lysate at 430 μg Primary: Anti-Angiotensin II type 1A receptor (TMAB-00079) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 41/51 kDa 4. Sample: Liver (Mouse) Lysate at 40 μg Lung (Mouse) Lysate at 40 μg Adrenal gland (Rat) Lysate at 40 μg Primary: Anti-Angiotensin II type 1A receptor (TMAB-00079) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 56 kDa 5. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (TMAB-00079) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. At least five transcript variants have been described for this gene. Additional variants have been described but their full-length nature has not been determined. The entire coding sequence is contained in the terminal exon and is present in all transcript variants. [provided by RefSeq]. |
| 免疫原 | KLH conjugated synthetic peptide: human Angiotensin II type 1A receptor |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cellular metabolic process,Angiogenic growth factors,Blood Pressure regulation,Cancer |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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