Anti-Acetyl-Histone H2A type 1-B/E (Lys9) Antibody (9N784) is an antibody targeting Acetyl-Histone H2A type 1-B/E (Lys9). Anti-Acetyl-Histone H2A type 1-B/E (Lys9) Antibody (9N784) can be used in ELISA, ICC, IF.

Rabbit IgG

9N784

Human

1. Immunocytochemistry analysis of TMAH-00007 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
2. Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with TMAH-00007 at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ELISA, ICC, IF

ICC:1:50-1:500; IF:1:30-1:200.

Monoclonal

Unconjugated

A synthetic peptide

Human

3012

Epigenetics and Nuclear Signaling