Powder: -20°C for 3 years | In solvent: -80°C for 1 year
TA01 是一种心源性抑制剂,有效抑制CK1和p38 MAPK,其对 CK1ε,CK1δ 和 p38 MAPK 的IC50值分别为 6.4 nM,6.8 nM 和 6.7 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 395 | 现货 | ||
2 mg | ¥ 582 | 现货 | ||
5 mg | ¥ 926 | 现货 | ||
10 mg | ¥ 1,630 | 现货 | ||
25 mg | ¥ 2,890 | 现货 | ||
50 mg | ¥ 4,280 | 现货 | ||
100 mg | ¥ 5,970 | 现货 | ||
500 mg | ¥ 12,500 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 996 | 现货 |
产品描述 | TA-01 is a potent CK1 and p38 MAPK inhibitor, with IC50s of 6.4 nM, 6.8 nM, 6.7 nM for CK1ε, CK1δ and p38 MAPK, respectively. |
靶点活性 | CKIδ:6.8 nM, p38 MAP kinase:6.7 nM, CKIϵ:6.4 nM |
体外活性 | TA-01 is a potent CK1 and p38 MAPK inhibitor, with IC50s of 6.4 nM, 6.8 nM, 6.7 nM for CK1ε, CK1δ and p38 MAPK, respectively. TA-01 (5 μM) is not cytotoxic, completely inhibits cardiogenesis, but induces cardiogenesis at lower concentration[1]. |
激酶实验 | Compounds (TA-01) are dissolved in DMSO?and tested at?10 μM concentrations?against a panel of 97 kinases, which are related to stem cell differentiation and cell signaling pathways. Kinome profiling is carried out by kinase profiling service[1]. |
细胞实验 | HES-3, H7 and IPS are harvested and seeded at 1.1 × 106 cells/mL as EBs in ultra-low attachment 12-well plates in bSFS medium: DMEM supplemented with 2 mM l-glutamine, 0.182 mM sodium pyruvate, 1% non-essential amino acids, 0.1 mM β-mercaptoethanol, 5.6 mg/L transferrin, 20 μg/L sodium selenite, 0.25% (w/vol) Bovine Serum Albumin and 0.25% (w/vol) Hysoy. Cells are incubated at 37°C and 5% CO2 to allow EB formation. The medium is refreshed after 1 day and then every 2-3 days. Cells are stimulated with the respective compounds (TA-01) dissolved in DMSO (1 μL DMSO/mL of media) starting from day 1 or day 4, until day 8. CHIR99021 is applied for the first 24 h only[1]. |
分子量 | 351.32 |
分子式 | C20H12F3N3 |
CAS No. | 1784751-18-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 9 mg/mL (25.62 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.8464 mL | 14.232 mL | 28.4641 mL | 71.1602 mL |
5 mM | 0.5693 mL | 2.8464 mL | 5.6928 mL | 14.232 mL | |
10 mM | 0.2846 mL | 1.4232 mL | 2.8464 mL | 7.116 mL | |
20 mM | 0.1423 mL | 0.7116 mL | 1.4232 mL | 3.558 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
TA-01 1784751-18-3 Autophagy MAPK Metabolism Stem Cells p38 MAPK Casein Kinase TA01 Inhibitor inhibit TA 01 inhibitor