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Anti-Phospho-p38 MAPK (Tyr323) Polyclonal Antibody
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产品编号 TMAB-01474
Anti-Phospho-p38 MAPK (Tyr323) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-p38 MAPK (Tyr323)。Anti-Phospho-p38 MAPK (Tyr323) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Phospho-p38 MAPK (Tyr323) Polyclonal Antibody
Anti-Phospho-p38 MAPK (Tyr323) Polyclonal Antibody
还可以产品编号 TMAB-01474
Anti-Phospho-p38 MAPK (Tyr323) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-p38 MAPK (Tyr323)。Anti-Phospho-p38 MAPK (Tyr323) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-Phospho-p38 MAPK (Tyr323) Polyclonal Antibody is a Rabbit antibody targeting Phospho-p38 MAPK (Tyr323). Anti-Phospho-p38 MAPK (Tyr323) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Horse,Rabbit) |
| 验证活性 | 1. Sample: Brain (Mouse) Lysate at 40 μg Primary: Anti-phospho-p38 MAPK (Tyr323) (TMAB-01474) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 45 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-p38 MAPK (Tyr323)) Polyclonal Antibody, Unconjugated (TMAB-01474) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse esophagus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-p38 MAPK (Tyr323)) Polyclonal Antibody, Unconjugated (TMAB-01474) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-p38 MAPK (Tyr323)) Polyclonal Antibody, Unconjugated (TMAB-01474) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases(MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| 免疫原 | KLH conjugated Synthesised phosphopeptide: human MAPK14 around the phosphorylation site of Tyr323 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | MAPK pathway,TLR Signaling,Other Cell Signaling Kits,MAPK Pathway |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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