2',7'-dichlorofluorescein

2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein) 是一种可渗透细胞的荧光探针，可用于检测一氧化氮和活性氧以及确定总体氧化应激的程度。

5(6)-Carboxy-2',7'-dichlorofluorescein，作为一种荧光素，被广泛应用于MRP2囊泡转运实验中，由于其出色的检测和转运特性，成为实验中的理想底物。

5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2’,7’,-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O’Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2’,7’-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

2,7-Dichlorodihydrofluorescein（DCFH,2,7-二氯二氢荧光素）是一种非荧光探针，被细胞内的活性氧（ROS）氧化后生成高荧光的2',7'-Dichlorofluorescein (DCF)。DCFH常用于检测细胞内的ROS水平，检测ROS的生成和分析氧化应激。

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester (cDCFDASE) 是一种用于检测细胞内 pH (pHin) 的荧光探针，特别适用于评估活细胞在中等 pH (pHex) 环境中的 pH 值。