SMAD5 Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 52.2 kDa and the accession number is P97454.
生物活性 | Testing in progress |
产品描述 | SMAD5 Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 52.2 kDa and the accession number is P97454. |
种属 | Mouse |
表达系统 | Baculovirus Insect Cells |
标签 | Tag Free |
蛋白编号 | P97454 |
别名 | MusMLP, AI451355, Madh5, Dwf-C, 1110051M15Rik, SMAD family member 5 |
蛋白构建 | The Mouse SMAD5 (P97454) (Thr2-Ser465) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 52.2 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM Nacl, 10% glycerol, pH8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. |
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SMAD5 Protein, Mouse, Recombinant MusMLP AI-451355 AI451355 Madh5 Dwf-C 1110051M15Rik AI 451355 SMAD family member 5 recombinant recombinant-proteins proteins protein