购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空

Nectin-3 Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPK-00319

The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein.

Nectin-3 Protein, Human, Recombinant (His & Avi), Biotinylated

Nectin-3 Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPK-00319
The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein.
规格价格库存数量
100 μg
¥ 5,180
5日内发货
500 μg
¥ 20,700
5日内发货
1 mg
¥ 34,600
5日内发货
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
如果低于说明书给出的最高溶解度情况下,产品仍未溶解怎么办?
建议您使用一些辅助溶解方法,如水浴加热至 45℃ 或用超声震荡,加速粉末溶解。
超声一般需要多久?
超声时长和溶解的量和浓度都有关,一般建议半个小时左右,如果浓度接近最大溶解度,或者需溶解的粉末较多,建议延长超声时间,并辅以加热。
动物不能耐受 DMSO,给药时 DMSO量应该如何控制?
对于普通的老鼠,DMSO 的浓度应控制在 10% 以下,对于裸鼠、转基因小鼠、耐受性弱的老鼠等,DMSO 浓度需控制在2%以下。对于首次操作的抑制剂,建议先做溶剂阴性对照,确认溶解对动物无非特异性影响。
PEG300 可以用 PEG400 替代吗?
PEG300 具有适度的粘稠度,有利于药物的溶解和给药过程,同时也能够提供良好的耐受性,不会对动物造成不良影响。PEG400 可以替代 PEG300。 PEG600 不是很建议,因为熔点接近常温。
如何稀释工作液?
根据您的工作液浓度,计算母液所需要稀释的倍数和所需母液的体积。建议用水、生理盐水、PBS等溶剂进行稀释,如果是细胞实验,可以使用培养基。 取适当的溶剂缓慢加入到母液中,直到达到您的工作液浓度,可以通过涡旋或者移液枪轻轻吹打帮助混匀。 我们的化合物大部分是脂溶性的,所以在使用 PBS 或者培养基等稀释工作液的时候,会有沉淀析出,这些都是正常现象,大多时候可以通过超声完全溶解。 工作液建议现配现用!
查看更多

产品信息

生物活性
Biotinylated Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.13 μM as determined in SPR assay.
产品描述
The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein.
种属
Human
表达系统
HEK293 Cells
标签C-His-Avi
蛋白编号Q9NQS3-1
别名
PVRR3,PVRL3,PRR3,PPR3,FLJ90624,CDw113,CD113
蛋白构建
Gly58-Asp400
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量40.6 kDa (predicted). Due to glycosylation, the protein migrates to 68-82 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 250 mM NaCl (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein.

参考文献

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.

关键词