Nectin-3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 39.3 kDa and the accession number is Q9NQS3-1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,820 | 5日内发货 | ||
1 mg | ¥ 24,900 | 5日内发货 |
生物活性 | Immobilized Human Nectin 3 His at 2 μg/mL (100 μL/well) can bind Human PVRL1/NECTIN1 hFc , the EC50 of Human PVRL1/NECTIN1 hFc is 3-24 ng/mL. |
产品描述 | Nectin-3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 39.3 kDa and the accession number is Q9NQS3-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q9NQS3-1 |
别名 | PRR3, FLJ90624, PVRL3, poliovirus receptor-related 3, CDw113, PPR3, CD113, PVRR3, DKFZp566B0846, nectin-3 |
蛋白构建 | The extracellular domain of Human PVRL3 (NP_056295.1) (Met 1-Asp 400) was expressed, with a polyhistidine tag at the C-terminus. |
蛋白纯度 | > 98 % as determined by SDS-PAGE |
分子量 | 39.3 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Poliovirus receptor-related 3 (PVRL3), also known as Nectin-3 and CD113, is a member of the nectin family. PVRL3/Nectin-3 is an 83 kDa, type I transmembrane glycoprotein. Its precursor is 549 amino acids (aa) in length and contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa, and a cytoplasmic region of 124 aa. Nectin-3 has three splicing variants, nectin-3alpha (biggest), -3beta (middle), and -3gamma (smallest). It is predominantly expressed in testis and placenta as well as in various cell lines, including epithelial cell lines. PVRL3/Nectin-3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/Nectin-2 at Sertoli-spermatid junctions. PVRL3/Nectin-3 is thus involved in the formation of cell-cell junctions, including adherens junctions and synapses. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in the reduction of cell movement and proliferation. |
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Nectin-3 Protein, Human, Recombinant (His) CD-113 FLJ 90624 PRR3 FLJ-90624 FLJ90624 PVRL3 DKFZp566B 0846 PRR 3 poliovirus receptor-related 3 CD 113 CDw113 DKFZp566B-0846 PPR 3 PPR-3 PPR3 PVRR-3 PVRL 3 PRR-3 PVRL-3 CD113 PVRR3 DKFZp566B0846 PVRR 3 nectin-3 recombinant recombinant-proteins proteins protein