购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空

IL-12RB2 Protein, Mouse, Recombinant (His)

IL-12RB2 Protein, Mouse, Recombinant (His)
产品编号 TMPY-01767

IL-12RB2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 70 kDa and the accession number is P97378.

IL-12RB2 Protein, Mouse, Recombinant (His)

IL-12RB2 Protein, Mouse, Recombinant (His)

IL-12RB2 Protein, Mouse, Recombinant (His)
产品编号 TMPY-01767
IL-12RB2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 70 kDa and the accession number is P97378.
规格价格库存数量
100 μg
¥ 3,820
现货
200 μg
¥ 6,750
5日内发货
500 μg
¥ 14,280
5日内发货
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
重组蛋白产品该如何保存?
1) 存储条件:重组蛋白产品收到后,如不立即使用,应当保存在 -20℃ 至 -80℃ 的条件下。冻干粉可稳定保存 12 个月以上,液体类产品在 -80℃ 条件下可保存 6-12 个月。已复溶的重组蛋白样品需尽快用完,如果不能在一周内用完,需在 -20℃ 至 -80℃ 保存,可保存 3 个月以上\n2) 避免反复融冻:重组蛋白产品应注意避免反复冻融,建议将重组蛋白产品分装成多份。分装冻存时,建议选择含有一定浓度载体蛋白的溶液或培养基复溶产品。产品浓度不低于 100 ug/mL,且每管的体积不小于 20 uL。
如何将冻干的重组蛋白配置为储备液?
在打开盖⼦之前,先使用 10000-12000 rpm 的转速进行离心,20-30 秒,将附着在管盖或管壁上的冻⼲粉收集到管底,以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂,产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL,分装储存避免反复冻融。
重组蛋白有吗?
您需要什么种属的蛋白呢,对表达系统和标签有要求吗,用于什么实验?
重组蛋白产品是如何运输的?
大部分重组蛋白产品为冻⼲粉,对温度不敏感,在室温下稳定,一般室温或蓝冰运输。对于液体类蛋白,一般采用⼲冰运输。如果您收到产品时发现冰盒已经融化,无需担心,蛋白可以正常使用,短时间内不会影响产品质量。如果特定蛋白在室温下不稳定,我们会提供相应的低温运输方式,以确保产品质量不受影响。
为什么重组蛋白的体积和质量与预期不符?
蛋白冻干粉由蛋白原液冻干而来,冻干粉质量不等同于蛋白质量。由于冻⼲产品的体积大⼩受多种因素影响,包括冻⼲前缓冲液的组分、盐离⼦浓度以及产品本身的浓度等。冻⼲粉可能看上去很多,也可能肉眼不易观察到,这些都是正常现象,请您放心使用。在收到产品后,照常复溶、使用或分装储存即可。
查看更多

产品信息

生物活性
Measured by its ability to bind Mouse IL12A & IL12B Heterodimer Protein in a functional ELISA. Immobilized mouse IL12RB2-His at 10 μg/ml (100 μl/well) can bind Mouse IL12A & IL12B Heterodimer Protein . The EC50 of Mouse IL12A & IL12B Heterodimer Protein is 50.2-117.2 ng/ml.
IL-12RB2 Protein, Mouse, Recombinant (His)
产品描述
IL-12RB2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 70 kDa and the accession number is P97378.
种属
Mouse
表达系统
HEK293 Cells
标签C-His
蛋白编号P97378
别名
interleukin 12 receptor, β2,interleukin 12 receptor, beta 2,IL-12RB2,Ifnm,A930027I18Rik
蛋白构建
A DNA sequence encoding the mouse IL12RB2 (NP_032380.1) extracellular domain (Met 1-Asn 637) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Asn 24
蛋白纯度
> 97 % as determined by SDS-PAGE
IL-12RB2 Protein, Mouse, Recombinant (His)
分子量70 kDa (predicted); 120-130 kDa (reducing condition, due to glycosylation)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Interleukin-12 receptor subunit beta-2 (IL12RB2), also known as IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, and IL-12RB2, is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. IL12RB2 belongs to the type I cytokine receptor family. The coexpression of IL12RB2 and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of IL12RB2 is up-regulated by IFN gamma in Th1 cells and plays a role in Th1 cell differentiation. The up-regulation of IL12RB2 is found to be associated with some infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. IL12RB2 promotes the proliferation of T-cells as well as NK cells. IL12RB2 induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

参考文献

技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.

关键词