TDCPP (Fyrol FR 2) is classified under the chlorinated trialkyl organophosphate flame retardant (OPFR) group of compounds primarily utilized as additives in commercial products.

Human corneal epithelial cells (HCECs) were cultured in DMEM supplemented with 10% FBS, 10 ng/mL EGF, and 1% penicillin-streptomycin solution in an incubator with 5% CO2 at 37℃.?After reaching confluence, HCECs were replanted into 6/24/ 96-well plates or petri dishes with different initial densities and cultured for 24 h to reach ~70% confluence.?Then, TDCPP dissolved in n-hexane was solvent-exchanged to DMSO and serially diluted by DMEM to achieve concentrations from 0.034 to 340 μg/mL.?For cell exposure, the culture medium was aspirated and treated with TDCPP solutions (DMSO <0.1%) for 24 h with 0.1% DMSO solution as vehicle control[1].