NCGC00188636 (also known as DBS) is a novel covalent pyruvate kinase (PYK) inhibitor. It exerts its effect by binding to the active site of pyruvate kinase and blocking nucleotide binding. The compound demonstrates potent inhibitory activity against Leishmania mexicana pyruvate kinase (LmPYK) and is used to investigate energy metabolism across various organisms and cell types.