Powder: -20°C for 3 years | In solvent: -80°C for 1 year
N-Salicyloyltryptamine(STP) 是一种色胺类似物,具有抗惊厥、抗炎、镇痛和血管舒张的作用。N-Salicyloyltryptamine 作为电压依赖性离子通道(包括Na+、Ca2+、K+离子通道)抑制剂,可抑制 K+电流,IC50值为 34.6 μM ( /to)。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 10,600 | 6-8周 | ||
50 mg | ¥ 13,800 | 6-8周 | ||
100 mg | ¥ 17,500 | 6-8周 |
产品描述 | N-Salicyloyltryptamine(STP) is a tryptamine analogue with anticonvulsant, anti-inflammatory, analgesic, and vasorelaxation effect. N-Salicyloyltryptamine acts as the voltage-dependent Na +, Ca 2+, and K + ion channels inhibitor which inhibits K + currents with an IC 50 value of 34.6 μM ( /to) [1] - [5]. |
体外活性 | N-Salicyloyltryptamine (1 ng/mL-1 μg/mL; 24 h) presents no cytotoxicity and causes no oxidative stress in RAW 264.7 cells at low concentration, but (50 and 100 μg/mL) inhibits cell viability with an IC 50 value of 22.75 μg/mL [1]. N-Salicyloyltryptamine (1 μg/mL; 24 h) reverses some redox and inflammatory parameters induced by LPS without interfering in cell viability [1]. N-Salicyloyltryptamine (1 μg/mL; 24 h) inhibits LPS-induced TNF-α and IL-1β release, as well as CD40 and TNF-α protein up-regulation [1]. N-Salicyloyltryptamine (1 μg/mL; 24 h) inhibits phosphorylation of ERK 1/2 and IκBα and p65 nuclear translocation (NF-kB activation) [1]. N-Salicyloyltryptamine (17 μM) inhibits K + current by 59.27% ( I to ) and 73.18% ( I KD ), inhibits L-type Ca 2+ currents by 54.9%, and shows few inhibition with high concentration (170 μM) on TTX-sensitive Na + current by 22.1% in GH3 cells [2]. N-Salicyloyltryptamine (0.01 nM-100 μM) produces vasorelaxation through activation of the NO/sGC/cGMP pathway and reduction of calcium influx [3]. Cell Viability Assay [1] Cell Line: RAW 264.7 cell Concentration: 0.001, 0.05, 1, 50, 100 μg/mL Incubation Time: 24 hours Result: Resulted no effect on RAW 264.7 cell viability at 1 μg/mL; however, concentrations of 50 and 100 μg/mL significantly decreased both MTT reduction and SRB incorporation. RT-PCR [1] Cell Line: RAW 264.7 cell Concentration: 1 μg/mL Incubation Time: 24 hours Result: Reduced CD40, TNF-α, and RAGE immunocontent. Inhibited ERK1/2 and IκBα phosphorylation and nuclear translocation of p65. |
体内活性 | N-Salicyloyltryptamine (100 mg/kg; i.p.; 60 min before stimulation challenge) significantly inhibits pentylenetetrazol (PTZ)-induced seizures and partially eliminates the extensor reflex of maximal electric-induced seizures test [4]. N-Salicyloyltryptamine (100 mg/kg, 200 mg/kg; i.p.; single dose) shows antinociceptive and nerve excitability effects [5]. Animal Model: Male Swiss mice (25-35 g) [4] Dosage: 50, 100, 200 mg/kg Administration: Intraperitoneal injection; single dose; 60 min before stimulation challenge Result: Reduced the incidence of clonic pentylenetetrazol (PTZ) seizures and mortality at 50 mg/kg, and decreased the incidence of tonic hindlimb extension (THE) produced by MES at 100, 200 mg/kg. Animal Model: Male Swiss mice (25-35 g) [5] Dosage: 100 mg/kg; 200 mg/kg Administration: Intraperitoneal injection; single dose Result: Reduced the acetic acid-induced licking response of the injected paw. |
分子量 | 280.32 |
分子式 | C17H16N2O2 |
CAS No. | 31384-98-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
N-Salicyloyltryptamine 31384-98-2 Inhibitor inhibitor inhibit