Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) is an antibody targeting Tri-methyl-Histone H4 (Lys20). Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) can be used in ELISA, WB, ICC.

Rabbit IgG

4H998

Human

1. Western Blot
-Positive WB detected in MCF-7 whole cell lysate, Mouse brain tissue,Mouse kidney tissue
-All lanes Tri-methyl-Histone H4 (K20) antibody at 2.15μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 11 KDa
-Observed band size: 11 KDa
2. Immunocytochemistry analysis of TMAH-01213 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

WB:1:500-1:5000; ICC:1:50-1:300.

Monoclonal

Unconjugated

A synthetic peptide

Human

Epigenetics and Nuclear Signaling